Part:BBa_K2707007:Design

Plasmid Tightly Regulated Copy-Control System

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 3548
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 4918
  Illegal BglII site found at 6986
  Illegal XhoI site found at 2753
  Illegal XhoI site found at 4159
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 4257
  Illegal NgoMIV site found at 4264
  Illegal NgoMIV site found at 4388
  Illegal NgoMIV site found at 5476
  Illegal NgoMIV site found at 9392
  Illegal AgeI site found at 1382
  Illegal AgeI site found at 5600
  Illegal AgeI site found at 6830
  Illegal AgeI site found at 7723
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 160
  Illegal SapI site found at 3540
  Illegal SapI site found at 6369
  Illegal SapI site found at 7579
  

Design Notes

if you need amplify this plasmid,you have to transfer it into E.coli EPI300

Source

CEN-ARS is from YAC pCC1BAC is from BAC